Persistence of fluoroquinolones and of ciprofloxacin resistant Enterobacteriacae in soil after poultry manure application
Persistance des Enterobacteriacae resistantes aux fluoroquinolones et à la ciprofloxacin dans le sol après épandage de fumier de poules
Dabert, P. ; Pourcher, A.M. ; Jadas Hécart, A. ; Cotinet, P. ; Ziebal, C. ; Le Roux, S. ; Moraru, R. ; Kempf, I.
Type de document
Communication scientifique avec actes
Affiliation de l'auteur
IRSTEA RENNES UR GERE FRA ; IRSTEA RENNES UR GERE FRA ; LETG ANGERS LEESA UMR 6554 CNRS ANGERS FRA ; CHAMBRE REGIONALE D'AGRICULTURE DE BRETAGNE RENNES FRA ; IRSTEA RENNES UR GERE FRA ; IRSTEA RENNES UR GERE FRA ; UNIVERSITATEA DE STIINTE AGRICOLES LASI ROU ; ANSES LABORATOIRE DE PLOUFRAGAN PLOUZANE PLOUFRAGAN FRA
Résumé / Abstract
This study aimed to evaluate the persistence (i) of two fluoroquinolones, enrofloxacin (ENR) and ciprofloxacin (CIP), and (ii) of CIP-resistant Enterobacteriaceae in soil after poultry manure application. The amounts of ENR and CIP in soil did not significantly change over the 48 day- experimental period. CIP (≤ 30 µg kg-1) was detected in weaker concentrations than ENR (20 to 250 µg kg-1 of soil). The concentration of Enterobacteriaceae (102-103 CFU g-1) did not significantly decrease until Day 36. A total of 145 isolates were identified as belonging to E. coli and to 6 genera. The ciprofloxacin MIC of E. coli reached 32 mg L-1 whereas the other strains of Enterobacteriaceae had a MIC ≤ 0.25 mg L-1. The 71 strains of E. coli were classified in 12 ERIC-PCR genotypes. One genotype, corresponding to a resistant strain, was detected until Day 89. This study showed that enrofloxacin and CIP-resistant E. coli persisted at least 48 and 89 days, respectively, in soil. However, their presence did not increase the MIC of Enterobacteriaceae originating from soil.
15th RAMIRAN International Conference. Recycling of organic residues for agriculture: from waste management to ecosystem services, 03/06/2013 - 05/06/2013, Versailles, FRA