European sturgeon (Acipenser sturio) is an anadromous fish which has been in sharp decline. Its last natural reproduction occurred in 1994. Because of global change, temperature have risen, frequency and severity of hypoxic events have increased. The goal of this paper is to determine the sensitivity of A. sturio early life stages to temperature and oxygen saturation. Embryo-larvae were exposed to combined temperature conditions, ranged from 12°C to 30°C, and dissolved oxygen, ranged from 30 to 90 % O2 saturation (% O2 sat). Acute and subacute effects were evaluated using embryonic and larval mortality, hatching success, malformation rate, routine metabolic rate (RMR) and swimming speed. Embryonic survival peaked at 20°C and no survival was recorded at 30°C. No hatching occurred at 50 % O2 sat or below. Malformation appeared to be minimum at 20°C, 90 % O2 sat. Maximum RMR were recorded at 20°C at 90 % O2 sat and 16°C at 70 % O2 sat. At 20°C, RMR at 70% O2 sat was lower than at 90% O2 sat. Swimming speed peaked at 16°C. A. sturio's temperature optimum is close to 20°C. Its upper tolerance limit is between 26 and 30°C and its lower tolerance limit is below 12°C. Oxygen depletion induce subacute effects at 70 % O2 sat and lethal effects at 50 % O2 sat. Current conditions in the basin are sustainable for A. sturio. Observing its sensitivity to oxygen depletion, a slight decrease would have large impact on European sturgeon survival capacity in the basin.